Purification and Characterization of Metallo-β-Lactamase fromSerratia marcescens
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چکیده
منابع مشابه
Purification and Biochemical Characterization of IMP-13 Metallo- -Lactamase
Metallo-lactamases (MBLs) are characterized by their ability to efficiently hydrolyze carbapenems and by their insensitivity to the commercially available -lactamase inhibitors (10). Several types of acquired MBLs have been described worldwide for Gram-negative nonfermenters (GNNFs), among which the IMPand VIM-type enzymes are the most widespread (9, 10). The IMP-13 MBL has 92.3% amino acid seq...
متن کاملEmergence of metallo-β-lactamase GIM-1 in a clinical isolate of Serratia marcescens.
The metallo-β-lactamase GIM-1 (German imipenemase) has been found so far only in clinical isolates of Pseudomonas aeruginosa from Germany. Here we report the detection of bla(GIM-1) in a clinical strain of Serratia marcescens that was isolated from urine, blood, and wound samples over a period of 20 months. The strain was repeatedly isolated from one patient in two German hospitals and an outpa...
متن کاملNew Delhi Metallo-β-Lactamase, Ontario, Canada
To the Editor: The New Delhi metallo-β-lactamase (NDM-1) was fi rst characterized in 2009 from Kleb-siella pneumoniae and Escherichia coli isolated from a patient in Sweden who had received medical care in New Delhi, India (1). Further studies have shown broad dissemination of this β-lactamase gene (bla NDM-1) in India, Pakistan, Bangladesh, and the United Kingdom (2). Additional isolates have ...
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Metallo-β-lactamase-producing Pseudomonas aeruginosa (MPPA) is an important nosocomial pathogen that shows resistance to all β-lactam antibiotics except monobactams. There are various types of metallo-β-lactamases (MBLs) in carbapenem-resistant P. aeruginosa including Imipenemase (IMP), Verona integron-encoded metallo-β-lactamase (VIM), Sao Paulo metallo-β-lactamase (SPM), Germany imipenemase (...
متن کاملBiochemical, Mechanistic, and Spectroscopic Characterization of Metallo-β-lactamase VIM-2
This study examines metal binding to metallo-β-lactamase VIM-2, demonstrating the first successful preparation of a Co(II)-substituted VIM-2 analogue. Spectroscopic studies of the half- and fully metal loaded enzymes show that both Zn(II) and Co(II) bind cooperatively, where the major species present, regardless of stoichiometry, are apo- and di-Zn (or di-Co) enzymes. We determined the di-Zn VI...
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ژورنال
عنوان ژورنال: Microbiology and Immunology
سال: 1995
ISSN: 0385-5600
DOI: 10.1111/j.1348-0421.1995.tb02164.x